Eppendorf Centrifuge 5424 R Service Manual [upd] Jun 2026

Clean rotor and bores. Apply a thin layer of anti-corrosion oil to anodized aluminum parts. Every 6 Months Centrifuge 5424 R - Eppendorf

The is a staple in molecular biology and clinical laboratories, renowned for its compact design, rapid cooling, and high-speed performance, reaching up to 21,130 ×gcross g

If you need to dive deeper into a specific technical issue with your unit, please let me know: The exact appearing on your display

Familiarity with the 5424 R's parts is the first step in any service work. The diagram below identifies some of its key features:

Before attempting any service, always refer to the most current official documentation. Eppendorf provides resources online at www.eppendorf.com . For everyday use, the covers general use and maintenance. Eppendorf Centrifuge 5424 R Service Manual

When the 5424 R encounters a malfunction, it will display an error code. Understanding these codes is essential for troubleshooting before calling for service. Error Code Potential Cause Action from Service Manual Speed sensor malfunction

To ensure safe and reliable operation, always follow these balancing rules:

Inspect the wiring harness connecting the motor to the main board. Check the speed sensor for physical debris or damage. Replace the sensor if it fails a continuity test. Error 5 (Lid Latch Error)

Detailed sections cover the PCB Display and PCB Control/Converter , which regulate all centrifuge processes. Clean rotor and bores

One of the most valuable sections of the 5424 R Service Manual is the . Here is a generalized procedure (always refer to the exact manual for your serial number range):

The 5424 R is more than just a spinning motor; it is a sophisticated system of integrated modules:

: Use pH-neutral detergents on the chamber, rotor, and adapters.

Detailed steps on replacing the rotor, ensuring the motor shaft is not damaged. The diagram below identifies some of its key

Wipe the rotor chamber with a neutral cleaning agent (pH 7-8) immediately if a spill occurs.

: For the refrigerated "R" model, regularly empty the condensation water tray located at the rear of the device. Technical Support

| Symptom / Message | Probable Cause | Recommended Action | | :--- | :--- | :--- | | | Rotor still spinning | Wait for the rotor to stop completely. | | | Power failure | 1. Check the device's mains fuse. 2. Check the laboratory's mains fuse. 3. Use the emergency release (see below). | | "LID ERROR" (Cannot lock) | Centrifuge lid cannot be locked | Try to close the centrifuge lid again. | | "LID ERROR" (Cannot release) | Centrifuge lid cannot be released | 1. Switch the device off and back on. 2. Press the open key. 3. If the error persists, use the emergency release. | | "LID LIFT" (5424 R only) | Lid not opened wide enough | Open the centrifuge lid wider by hand. | | "Err 6" / Drive error | Drive error | Repeat the run. If the error appears again, switch the centrifuge off and back on after a 20-second delay. | | | Drive overheated | Allow the drive to cool down for at least 15 minutes. | | "Err 21" (5424 R only) | Temperature sensor at capacitor faulty | Switch the centrifuge off and back on again after a 20-second delay. | | Device does not start | Lid not closed properly | Close the centrifuge lid. | | Device jerks at start | Rotor loaded asymmetrically | Stop the device, load the rotor symmetrically, and restart. | | "INT" (Mains power failure) | Power failure during a run | Check the mains connection. | | "NO RPM" | Error in speed measuring system | Leave the device switched on until the error message disappears (10 seconds or 6 minutes). | | Short run stops | "Short" key released more than twice | Keep the "short" key pressed down during the short centrifugation run. | | Temperature display flashes (5424 R only) | ΔT > 3 °C deviation from set value | Check the settings and ensure the ventilation slots are unobstructed. |