Search "Shimadzu SPD-20A lamp replacement" on YouTube’s Shimadzu Scientific Instruments channel. These videos align exactly with the manual’s written steps but include visual guidance.
Check the lamp intensity; it may be time to replace the lamp. Also, check for air bubbles in the flow cell.
Contaminated flow cells cause baseline drift and ghost peaks. Flush the cell with HPLC-grade water to remove salts. Flush with 1M Nitric Acid ( HNO3cap H cap N cap O sub 3 ) if organic fouling is severe.
. The "Remote" LED should light up if connected to software like EZStart or LabSolutions. shimadzu spd20a user manual
Ensure all connections are made and the cell is filled with mobile phase (never turn the lamp on with a dry cell). Turn on the main power switch.
This informative paper is intended for educational and quick-reference purposes only. Always consult the original manufacturer manual before performing maintenance or repairs.
The manual warns never to turn on the deuterium lamp without the flow cell installed—you will permanently damage the optical system. Also, check for air bubbles in the flow cell
To prevent downtime, users should adhere to the following maintenance schedule:
Accesses validation and parameter configuration submenus.
Set the desired detection wavelength (e.g., 280 nm for protein analysis). Flush with 1M Nitric Acid ( HNO3cap H
for the lamp to cool completely to avoid burns. Remove the lamp compartment cover screws. Disconnect the lamp power connector. Loosen the two retaining screws holding the lamp assembly.
I can provide targeted troubleshooting steps for your exact laboratory scenario. Share public link
Allow the lamp to warm up for to stabilize the baseline. Step 2: Setting Parameters
Check for air bubbles in the flow cell. Perform lamp energy checks to see if the Deuterium lamp is failing.
I can’t provide or reproduce the full user manual (copyrighted material) for the Shimadzu SPD-20A. I can, however, help with any of the following: