Manual New: Narishige Pc10

The PC-10 is a vertical, two-stage puller designed to create sharp micropipettes with consistent tip diameters and tapers. Its vertical design utilizes gravity, reducing the influence of friction compared to horizontal pullers, which helps in achieving highly reproducible results.

In the shadowy corners of neuroscience labs and electrophysiology workbenches, one name whispers precision: . And among their legendary tools, the PC-10 micropipette puller holds a cult status. But there’s a problem—a quiet crisis brewing on used equipment shelves and in university storage rooms.

Choosing your mode depends on your experimental requirements:

Secure the top clamp first, ensure the glass hangs perfectly vertical, and then tighten the bottom clamp. Step 4.2: Configuring Parameters

Narishige PC-10 is a vertical micropipette puller that uses gravity and automated two-stage pulling to create glass needles for microinjection and patch clamping. Tritech Research Key Controls & Components Mode-Selector Knob : Switches between four modes: : Starts single-stage pulling. : Starts two-stage (double) pulling. NO. 1 HEATER / NO. 2 HEATER narishige pc10 manual new

List the when pulling thin-walled glass.

Attach the weight (if not already attached) to the lower holding assembly. 3. The PC-10 Operation Mechanism

Rotate the bottom switch 90 degrees counterclockwise to the "under weight" position (if applicable to your version). Press the red Start Switch

The updated Narishige PC-10 manual includes the following new features: The PC-10 is a vertical, two-stage puller designed

Because every great micropipette begins with a great manual.

The most critical section of the PC-10 manual is the calibration protocol. This includes:

What is the of your glass capillaries?

Loosen clamps and re-center the glass perfectly within the loop. Broken filament or loose contact. And among their legendary tools, the PC-10 micropipette

While simple in design, this system allows you to preset two different heating levels—one for the initial stretch and another for the final pull—enabling the production of everything from long, thin microinjection needles to firm, blunt microelectrodes for patch-clamping. Key Specifications & Features

However, there is a persistent problem: the original paper manuals are often lost, damaged by chemical spills, or written in dense technical Japanese. This leads to a frantic search for a new Narishige PC-10 manual—not necessarily a brand-new machine, but a fresh, readable, and complete version of the documentation.

Since the PC-10 is discontinued, you will not find a "new" manual for the original model. However, the official documentation is still available. The best resources for finding "new" information are the :

We use this primarily for standard patch-clamp electrodes (borosilicate glass, 1.5mm OD).

Research communities can be excellent sources of practical knowledge. For example, the Alliance of Genome Resources forum has a discussion where a user describes their settings: "We use the PC-10 capillary puller (step 1 mode, 59 C) with the Narishige GD-1 capillaries. We center the capillaries to get two needles out of each". While not a full manual, these notes offer empirical calibration data. Dedicated forums can uncover real-world notes and tips from other users.